Down-regulation of CD147 expression induces SiHa cell apoptosis through the Bcl-2 pathway.
- Author:
Xi ZHOU
1
;
Jie-yu LV
;
Bao-lan GONG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Apoptosis Regulatory Proteins; metabolism; Basigin; metabolism; Bcl-2-Like Protein 11; Caspase 3; metabolism; Cell Line, Tumor; Down-Regulation; Humans; Membrane Proteins; metabolism; Proto-Oncogene Proteins; metabolism; Proto-Oncogene Proteins c-bcl-2; metabolism; RNA Interference
- From: Journal of Southern Medical University 2010;30(7):1695-1698
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in SiHa cell apoptosis after inhibition of CD147 expression.
METHODSRNA interference (RNAi) technique was used to down-regulate CD147 expression in SiHa cells, and RT-PCR and Western blotting were used to detect expression of CD147, Bcl-2, Bim and caspase-3; the percentage of cell apoptosis were detected by flow cytometry.
RESULTSSiRNA sequence 1, 2 inhibited CD147 expression in SiHa cells effectively (P<0.05), resulting also in down-regulated expression of Bcl-2 (P<0.05) and up-regulated expression of caspase-3 and Bim(P<0.05). The percentage of apoptotic cells increased significantly, and early apoptosis was the most obvious in the cells (P<0.05).
CONCLUSIONSilencing of CD147 expression induces SiHa cell apoptosis partially through the Bcl-2 pathway .
