Protein kinase D3 is involved in negative regulation of MMP-7 in prostate cancer cells.
- Author:
Zhi-peng ZOU
1
;
Li FENG
;
Wan-fu XU
;
Zhi-yong KE
;
Q Jane WANG
;
Fan DENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Down-Regulation; Gene Knockdown Techniques; Humans; Male; Matrix Metalloproteinase 7; metabolism; Prostatic Neoplasms; enzymology; metabolism; Protein Kinase C; metabolism; Signal Transduction
- From: Journal of Southern Medical University 2010;30(8):1767-1770
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of protein kinase D3 (PKD3) in the regulation of matrix metalloproteinases 7 (MMP-7) expression in prostate cancer cells.
METHODSPC-3 cells were either stimulated with 100 nmol/L PMA to activate PKD3 kinase activity, or transiently transfected with PKD3 siRNA, and the relative expression level of MMP-7 mRNA were analyzed by real-time PCR using 2(-delta delta Ct) method. MMP-7 mRNA levels were also analyzed and quantified in HEK293 cells with over-expression of wild-type PKD3, PKD3 knockdown (using PKD3 siRNA), or over-expression of wild-type PKD3 followed by PKD3 knockdown.
RESULTSMMP-7 mRNA expression in PC3 cells was significantly decreased after PMA-induced PKD3 kinase activation. In contrast, PKD3 knockdown by siRNA transfection markedly increased MMP-7 mRNA level (P<0.01). MMP-7 mRNA level in HEK293 cells was significantly decreased by PKD3 over-expression, whereas obviously increased by PKD3 knockdown. Down-regulation of MMP-7 mRNA level in HEK293 induced by PKD3 over-expression was rescued by PKD3 knockdown.
CONCLUSIONPKD3 may contribute to the malignant progression of prostate cancer cells through negative regulation of MMP-7 expression.
