Effect of NAD+ against radiation injury and its dose-effect relationship.
- Author:
Min-ying LI
1
;
Feng LEI
;
Wei YOU
;
Yu-jing TAN
;
Xiao-jun LU
;
Long-hua CHEN
;
Ji-ren ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; radiation effects; Cell Line; Cell Survival; drug effects; radiation effects; Dose-Response Relationship, Drug; Hepatocytes; cytology; drug effects; radiation effects; Humans; NAD; administration & dosage; pharmacology; Radiation Injuries; prevention & control
- From: Journal of Southern Medical University 2010;30(8):1787-1789
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of NAD+ against radiation injury and its dose-effect relationship.
METHODSL02 liver cells cultured in RPMI 1640 medium containing 10% fetal calf serum were exposed to X-ray irradiation followed by immediate application of NAD+. The cellular viability was analyzed by MTT assay and the apoptotic cells were detected by TUNEL methods to observe the damages of L02 liver cells induced by X-ray exposure and analyze the dose-effect relationship of NAD+.
RESULTSThe viability of L02 liver cells was decreased with increasing dose of X-ray irradiation. The most obvious growth inhibition of L02 cells occurred 24 h after the irradiation. NAD+ significantly increased the cell survival rate after irradiation, and this effect was gradually increased within the concentration range of 100-1000 microg/ml; at higher concentrations, the survival rate of the irradiated L02 cells showed no significant increase.
CONCLUSIONNAD+ provides partial protection of the liver cells against radiation injury, and the effect is positively correlated to NAD+ concentration within a certain range.
