Effects of radiosensitivity and X-ray dose on miR-7 expression in nasopharyngeal carcinoma.
- Author:
Zhi-xian CHEN
1
;
Ai-min SUN
;
Yong CHEN
;
Ying LIU
;
Jun-fang ZHAN
;
Long-hua CHEN
;
Ya-wei YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; radiation effects; Carcinoma; Cell Line, Tumor; Dose-Response Relationship, Radiation; Gene Expression Regulation, Neoplastic; radiation effects; Humans; MicroRNAs; genetics; Nasopharyngeal Neoplasms; genetics; Radiation Tolerance; genetics; X-Rays
- From: Journal of Southern Medical University 2010;30(8):1810-1816
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of radiosensitivity and X-ray dose on the expression of miR-7 in nasopharyngeal carcinoma (NPC) cells.
METHODSLow radiosensitive NPC cells CNE-1 and high radiosensitive NPC cells CNE-2 were exposed to 0, 2 and 8 Gy X-ray. The total RNAs of the cell lines were extracted 10 h after radiation for reverse transcription of miR-7 and 18S rRNA by stem-loop primer and random hexamers, respectively. The non-irradiated CNE-1 cells served as the control sample and the relative quantity of the expression level was calculated after real-time PCR using SyBR green.
RESULTSmiR-7 expression differed significantly between CNE-1 and CNE-2 cells (4.49-/+3.62 vs 1.29-/+1.10, F=135.483, P<0.001). The radiation dose also significantly affected the expression of miR-7 in NPC cells (F=39.565, P<0.001). CNE-1 cells with a 2 Gy exposure had the highest expression level of miR-7, while the non-irradiated CNE-1 cells had the lowest expression. CNE-2 cells exposed to 2 Gy X-ray had the lowest expression level of miR-7 and the non-irradiated CNE-2 cells had the highest.
CONCLUSIONRadiosensitivity and radiation dose of X-ray have significant effect on the expression of miR-7 in NPC cells, indicating that miR-7 plays an important role in radioresistance of NPC cells to X-ray, and suppressed miR-7 expression may elevate the radiosensitivity of NPC cells.
