Norcantharidin inhibits DNA replication initiation protein Cdc6 in cancer cells.
- Author:
Jin-long LI
1
;
Yu-chen CAI
;
Zhi-ming HU
;
Ji-min GAO
Author Information
- Publication Type:Journal Article
- MeSH: Bridged Bicyclo Compounds, Heterocyclic; pharmacology; Cell Cycle Proteins; metabolism; Cell Line, Tumor; DNA Replication; drug effects; Humans; Nuclear Proteins; metabolism
- From: Journal of Southern Medical University 2010;30(8):1851-1853
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the inhibitory effect of norcantharidin (NCTD) on the expression of DNA replication initiation protein Cdc6 in cancer cells.
METHODSMTT assay was performed to detect the inhibitory effect on different cancer cell lines, including HeLa, HepG2, Jurkat and Ramos cells. The effect of NCTD on Cdc6 protein level was detected by Western blotting, and BrdU incorporation assay was used to evaluate the DNA replication of the cells.
RESULTSNCTD significantly inhibited the proliferation of the cells and caused degradation of Cdc6 protein to result in the inhibition of the DNA replication of the cells shown by BrdU incorporation assay.
CONCLUSIONNCTD can induce the degradation of Cdc6 in cancer cells to produce an anti-cancer effect.
