Expression of uncoupling protein 2 on macrophages and its relation to interleukin-10/interferon ratio in patients with unexplained recurrent spontaneous abortion.
- Author:
Zhi-hong FU
1
;
Wen-jie ZHU
;
Xiu-min CHEN
;
Xue-mei LI
;
Zhen TAN
;
Yong-hong ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Abortion, Habitual; metabolism; Adult; Cytokines; metabolism; Decidua; cytology; metabolism; Female; Humans; Interferon-gamma; secretion; Interleukin-10; secretion; Ion Channels; metabolism; Macrophage Activation; Mitochondrial Proteins; metabolism; Pregnancy; Uncoupling Protein 2
- From: Journal of Southern Medical University 2010;30(8):1854-1856
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between the expression of uncoupling protein 2 (UCP2) and ratio of interleukin-10/interferon-gamma (IL10/IFNgamma) in the macrophages from patients with unexplained recurrent spontaneous abortion (URSA).
METHODSTwelve women undergoing selective termination of normal early pregnancy (control) and 11 having URSA were included in this study. Magnetic cell sorting (MACS) was used to isolate the macrophages in the decidua, and the expression of UCP2 was detected with flow cytometry. Cytokine (IL10 and IFNgamma) secretion by the macrophages was detected by enzyme-linked immunosorbent spot-forming (ELISPOT) cell assay.
RESULTSCompared to the control group, the women with URSA showed significantly decreased expression of UCP2 on decidual macrophage (136-/+25 vs 201-/+31, P<0.01), and the expression of UCP2 was positively correlated to the ratio of IL10/IFNgamma(r=0.73, P<0.01).
CONCLUSIONUCP2 may play an important role in the regulation of macrophage activity and cytokine secretion to contribute to spontaneous abortion.
