Construction of recombinant adenovirus containing TK gene and its effect against human liver cancer cells.
- Author:
Geng-zhen CHEN
1
;
Hui HU
;
Ming-yan XU
;
Xiao-ling DENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Cell Line, Tumor; Genetic Therapy; Genetic Vectors; Humans; Liver Neoplasms; therapy; Thymidine Kinase; genetics
- From: Journal of Southern Medical University 2010;30(8):1887-1889
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a replication-defective adenovirus containing TK gene and investigate the killing effects of TK gene against human liver cancer cells SMMC-7721.
METHODSThe recombinant adenovirus ADV-TK was constructed using homologous recombination in the cells. SMMC-7721 cells transfected with recombined adenovirus were exposed to GCV, and the cell viability was measured by MTT assays.
RESULTSThe recombinant adenovirus containing TK gene was successfully constructed. Transfection by the recombinant adenovirus ADV-TK and GCV exposure significantly suppressed the growth of SMMC-7721 cells.
CONCLUSIONA replication-defective adenovirus containing TK gene has been successfully constructed, and in combination with GCV, the recombinant adenovirus produces significant killing effect against SMMC-7721 cells in vitro.
