Application of immunohistochemical labeling in the diagnosis of hepatobiliary intraepithelial neoplasia and/or cancer.
- Author:
Yan-chun LI
1
;
Hong-mei YI
;
Ren-hua ZHONG
;
Zhuo-ri LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Antibodies, Monoclonal; Antigens, Neoplasm; analysis; Bile Duct Neoplasms; diagnosis; pathology; Bile Ducts, Intrahepatic; pathology; Carcinoma in Situ; diagnosis; pathology; Epithelium; pathology; Female; Humans; Immunohistochemistry; Male; Middle Aged
- From: Journal of Southern Medical University 2010;30(8):1909-1911
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the best combination of monoclonal antibodies for the diagnosis of hepatobiliary intraepithelial neoplasia and/or cancer.
METHODSCK7, CK20, Villin, CEA, P53 and Ki-67 antigens were detected in the tissues of high-level hepatobiliary intraepithelial neoplasia and cancer by immunohistochemistry and the results were analyzed statistically.
RESULTSVillin was 100% positive in hepatobiliary intraepithelial neoplasia and/or cancer, while 100% negative in the adjacent normal bile duct epithelium. The expression rate of CEA was significantly lower in high-level hepatobiliary intraepithelial neoplasia tissues than in the cancer tissues (P<0.05). Ki-67 indexes were significantly lower in most of the high-level hepatobiliary intraepithelial neoplasia than in the cancer tissue (P<0.01). P53 indexes were also lower in high-level hepatobiliary intraepithelial neoplasia (P<0.01).
CONCLUSIONDetection of multiple antigens (CEA, Villin, Ki-67 and P53) provides specific clues to the diagnosis of high-grade hepatobiliary intraepithelial neoplasia and/or cancer.
