Construction and identification of blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid.
- Author:
Min LUO
1
;
Xu-feng LI
;
Dong-zhi CEN
;
Jian-jun ZOU
;
Ji-ren ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Blood Group Antigens; genetics; Cell Line, Tumor; Gene Expression; Humans; Macrophages; Peptides; genetics; Plasmids; Recombinant Fusion Proteins; genetics; Recombinant Proteins; genetics
- From: Journal of Southern Medical University 2010;30(8):1939-1942
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct and identify blood type B antigen mimetic polypeptide-macrophage inflammatory protein 3beta (Mip3beta) double expression recombinant plasmid.
METHODSThe positive phage clone P1 was obtained using phage random 12-mer peptide library. Specific primers were designed to amplify the phage DNA of P1 and transmembrane domain and inner segment of PBluscript-Fas gene. The products of the amplification were linked into Mip3betav21 to construct blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid. The recombinant plasmid was transfected into human melanoma cell line B16 to identify its expression.
RESULTS AND CONCLUSIONBlood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid is successfully obtained and expressed in human melanoma cell line B16.
