Expression of topoisomerase II alpha in human colorectal carcinoma and its significance.
- Author:
Fa-da YANG
1
;
Zhang-lin JIA
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Neoplasm; metabolism; Colorectal Neoplasms; metabolism; pathology; DNA Topoisomerases, Type II; metabolism; DNA-Binding Proteins; metabolism; Female; Humans; Lymphatic Metastasis; Male; Poly-ADP-Ribose Binding Proteins
- From: Journal of Southern Medical University 2010;30(8):1959-1964
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the correlation between the expression of topoisomerase 2 alpha (TOP2A) and the biological behaviors of human colorectal carcinoma.
METHODSImmunohistochemistry and real-time RT-PCR were used to detect the expression of TOP2A in colorectal carcinomas and normal mucosa.
RESULTSThe protein and mRNA expressions of TOP2A in the metastatic lymph nodes were significantly higher than those in matched primary lesions and normal tissues (P<0.05). No significant difference was found in TOP2A expressions between normal mucosa and colorectal carcinomas. The protein and mRNA expressions of TOP2A were significantly correlated to the lymph node metastasis and invasion depth (P<0.05), but not to the differentiation of the tumor (P>0.05).
CONCLUSIONTOP2A plays an important role in the invasion and metastasis of the colorectal carcinomas, and may serve as a valuable indicator for the diagnosis, treatment and the prognostic evaluation of the malignancy.
