Role of Na(+)-K(+)-ATPase in lipopolysaccharide-induced cardiomyocyte hypertrophy in rats.
- Author:
Li-qun WANG
1
;
Tang-ting CHEN
;
Ying-qian CAI
;
Xiang XUE
;
Xiang ZHOU
;
Chun-hua JIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Enlargement; drug effects; Cells, Cultured; Hypertrophy; chemically induced; Lipopolysaccharides; Myocytes, Cardiac; enzymology; pathology; Rats; Rats, Wistar; Sodium-Potassium-Exchanging ATPase; metabolism
- From: Journal of Southern Medical University 2010;30(9):2059-2062
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the possible mechanism of lipopolysaccharide (LPS)-induced cardiomyocyte hypertrophy in rats.
METHODSNeonatal rat cardiomyocytes cultured in vitro were stimulated with 100 µg/L LPS for 1, 4 or 8 h and scanned by atomic force microscopy (AFM) for measurement of the two-dimensional area, three-dimensional surface area and volume of each cell. The total proteins and Na(+)-K(+)-ATPase activity in the cardiomyocytes were determined. The same measurements were also carried out in neonatal rat cardiomyocyte cultures stimulated by 0.5 µmol/L ouabain for 8 h and the total protein levels were measured.
RESULTSFollowing a 8-hour stimulation with LPS, the two-dimensional area, three-dimensional surface area and volume of the single cardiomyocyte became enlarged and the total cellular proteins increased significantly as compared with those in the normal control cells (P < 0.05). LPS treatment for 4 and 8 h resulted in significantly decreased activity of Na(+)-K(+)-ATPase in the cardiomyocytes (P < 0.05). In the cells treated with ouabain for 8 h, the two-dimensional area, three-dimensional surface area, volume of the single cardiomyocyte and the total cellular proteins increased significantly in comparison with the normal control group (P < 0.05).
CONCLUSIONLPS can result in cardiomyocyte hypertrophy in rats possibly in relation to lowered Na(+)-K(+)-ATPase activity in the cardiomyocytes after LPS exposure.
