Hemocompatibility evaluation in vitro of small-caliber expanded polytetrafluoroethylene vessel with silk fibroin coating sulfonated by low temperature plasma.
- Author:
Shao-bin LI
1
;
Yu-sheng YAN
;
Hui LI
;
Kun-tang CHEN
;
Jian TONG
;
Qun-qing CHEN
;
Fu-wei ZHANG
;
Fei XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Blood Vessel Prosthesis; Coated Materials, Biocompatible; chemistry; Cold Temperature; Fibroins; chemistry; Humans; Materials Testing; Plasma Gases; Polytetrafluoroethylene; chemistry; Sulfonic Acids; chemistry
- From: Journal of Southern Medical University 2010;30(9):2100-2103
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the hemocompatibility of a small-caliber expanded polytetrafluoroethylene vessel with silk fibroin coating sulfonated by low temperature plasma treatment.
METHODSThe composite blood vessel was prepared by first coating the small-caliber expanded polytetrafluoroethylene vessel with silk fibroin followed by sulfonation by low temperature plasma treatment. After hemolysis test in vitro, dynamic coagulation time test, blood platelet adhesion test, and recalcification time test were performed to evaluate the hemocompatibility of the composite blood vessel.
RESULTSScanning electronic microscopy revealed obvious platelets adhesion on the conventional artificial (control) vessel, which seldom occurred on the composite vessel. The curve of absorbance-clotting time of the composite vessel declined more slowly than that of the control vessel. The recalcification time of the composite blood vessel averaged 603 s, significantly longer than that of the control vessel (480 s, P = 0.000).
CONCLUSIONThe composite blood vessel has good antithrombotic activity and hemocompatibility as a promising vascular prosthesis.
