Relationship of NOR-1 with the regulation of inflammation via liver X receptor alpha in Kupffer cells.
- Author:
Zhuo-ya DAI
1
;
Jian-ping GONG
;
Si-dong WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; DNA-Binding Proteins; metabolism; Inflammation; metabolism; Interleukin-10; metabolism; Kupffer Cells; metabolism; Liver X Receptors; Male; Mice; Mice, Inbred Strains; Nerve Tissue Proteins; metabolism; Orphan Nuclear Receptors; metabolism; Receptors, Steroid; metabolism; Receptors, Thyroid Hormone; metabolism; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Hepatology 2011;19(7):542-546
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship of NOR-1 with the inhibition of inflammatory reaction in mice Kupffer cells (KCs) induced by lipopolysaccharide (LPS) via liver X receptor alpha (LXR alpha).
METHODSKCs from male KM mice were isolated by density gradient centrifugation, incubated and then randomly assigned to three groups: control group, LPS treated group and LPS+T0901317 treated group.
RESULTSThe mRNA and protein expressions of LXR alpha and NOR-1 in each group were determined by RT-PCR, immunofluorescent assay and western blot, respectively. The densities of TNF alpha and IL-10 in supernatants were evaluated by enzyme linked immunosorbent assay (ELISA). The mRNA and protein expression levels of LXR alpha in LPS + T0901317 group were the highest as compared to the other two groups (0.748+/-0.072 and 1.217+/-0.133 respectively), The mRNA and protein expression levels of NOR-1 in LPS+ T0901317 group were the highest as compared to the other two groups (2.726+/-0.065 and 0.842+/-0.058 respectively). The densities of supernatant TNF alpha in LPS group and IL-10 in LPS+T0901317 group were the highest [(450.89+/-78.52) ng/L and (537.41+/-36.41) ng/L respectively].
CONCLUSIONSPromoting the expression of LXR alpha in KCs can elevate the NOR-1 expression and then inhibit inflammatory reaction.
