Wnt/b-catenin signaling pathway affects the protein expressions of caspase-3, XIAP and Grp-78 in hepatocellular carcinoma.
10.3760/cma.j.issn.1007-3418.2011.08.010
- VernacularTitle:肝癌细胞中Wnt/β-连环素信号通路与caspase-3、X连锁凋亡抑制蛋白和葡萄糖调节蛋白78的表达
- Author:
Xin-Hong WANG
1
;
Xiang-Wei MENG
;
Xun SUN
;
Ya-Ju DU
;
Jing ZHAO
;
Yu-Jing FAN
Author Information
1. Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130012, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma, Hepatocellular;
Caspase 3;
Catenins;
Humans;
Liver Neoplasms;
Wnt Signaling Pathway;
beta Catenin;
metabolism
- From:
Chinese Journal of Hepatology
2011;19(8):599-602
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the relationship and significance of Wnt/b-catenin signaling pathway with caspase-3, XIAP, HSP27and Grp-78. The HCC cell line HepG2 was transfected with small interfering RNA (siRNA) directed against b-catenin. After 72 and 96 h, protein was extracted and the protein expressions of b-catenin, caspase-3, XIAP, Grp-78 and HSP27 were detected by Western blot. b-catenin protein expression was inhibited at both time points and the expression at 96 h was higher than that at 72 h (F = 160.72, P is less than to 0.01). Interestingly, Caspase-3 protein expression was decreased at 72 h and increased to normal at 96 h (F = 136.10, P is less than to 0.01), while p-caspase-3 protein expression increased at 72 h and decreased to normal at 96 h (F = 98.65, P is less than to 0.01). XIAP protein expression decreased at 72 h (F = 37.29, P is less than to 0.01) and increased at 96 h. Grp-78 protein expression increased at 72 h and decreased to normal at 96 h ( F = 58.72, P is less than to 0.01). HSP27 protein expression showed no change following transfection ( F = 1.91, P is more than to 0.05). Wnt/b-catenin signaling pathway is related to the protein expressions of caspase-3, XIAP and Grp-78, but not related to HSP27 protein expression in HCC. Wnt/b-catenin signaling pathway may participate in the regulation of HCC apoptosis, proliferation and differentiation through affecting these factors.
