Rhubarb Monomers Protect Intestinal Mucosal Barrier in Sepsis via Junction Proteins.

Lyu Wang; Yun-liang Cui; Zhe Zhang; Zhao-fen Lin; De-chang Chen

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Rhubarb Monomers Protect Intestinal Mucosal Barrier in Sepsis via Junction Proteins.

Author: Lyu WANG ¹ ; Yun-Liang CUI ² ; Zhe ZHANG ¹ ; Zhao-Fen LIN ¹ ; De-Chang CHEN ³
Author Information

1. Department of Emergency and Critical Care Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
2. Department of Critical Care Medicine, Jinan Military General Hospital, Jinan, Shandong 250031, China.
3. Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University, Shanghai 200025, China.
Publication Type:Journal Article
MeSH: Animals; Claudin-5; metabolism; Intestinal Mucosa; drug effects; metabolism; Lactulose; metabolism; Male; Mannitol; metabolism; Occludin; metabolism; Plant Extracts; chemistry; therapeutic use; Rats; Rats, Sprague-Dawley; Rheum; chemistry; Sepsis; drug therapy; metabolism; Zonula Occludens-1 Protein; metabolism
From: Chinese Medical Journal 2017;130(10):1218-1225
CountryChina
Language:English
Abstract:
BACKGROUNDLeakage of the intestinal mucosal barrier may cause translocation of bacteria, then leading to multiorgan failure. This study hypothesized that rhubarb monomers might protect the gut mucosal barrier in sepsis through junction proteins.
METHODSHealthy male Sprague-Dawley rats (weighing 230-250 g) under anesthesia and sedation were subjected to cecal ligation and perforation (CLP). After surgical preparation, rats were randomly assigned to eight groups (n = 6 or 8 each group): sham group (Group A: normal saline gavage); sepsis group (Group B: normal saline gavage); Group C (intraperitoneally, dexamethasone 0.5 mg/kg) immediately after CLP surgery; and rhubarb monomer (100 mg/kg in normal saline)-treated groups (Group D: rhein; Group E: emodin; Group F: 3,8-dihydroxy-1-methyl-anthraquinone-2-carboxylic acid; Group G: 1-O-caffeoyl-2-(4-hydroxy-O-cinnamoyl)-D-glucose; and Group H: daucosterol linoleate). Animals were sacrificed after 24 h. Intestinal histology, lactulose, mannitol concentrations were measured, and zonula occludens (ZO)-1, occludin and claudin-5 transcription (polymerase chain reaction), translation (by Western blot analysis), and expression (by immunohistochemistry) were also measured.
RESULTSIntestinal histology revealed injury to intestinal mucosal villi induced by sepsis in Group B, compared with Group A. Compared with Group A (0.17 ± 0.41), the pathological scores in Groups B (2.83 ± 0.41, P < 0.001), C (1.83 ± 0.41, P < 0.001), D (2.00 ± 0.63, P < 0.001), E (1.83 ± 0.41, P < 0.001), F (1.83 ± 0.75, P < 0.001), G (2.17 ± 0.41, P < 0.001),and H (1.83 ± 0.41, P < 0.001) were significantly increased. Lactulose/mannitol (L/M) ratio in Group B (0.046 ± 0.003) was significantly higher than in Group A (0.013 ± 0.001, P< 0.001) while L/M ratios in Groups C (0.028 ± 0.002, P< 0.001), D (0.029 ± 0.003, P< 0.001), E (0.026 ± 0.003, P< 0.001), F (0.027 ± 0.003, P< 0.001), G (0.030 ± 0.005, P< 0.001), and H (0.026 ± 0.002, P< 0.001) were significantly lower than that in Group B. ZO-1, occludin and claudin-5 transcription, translation, and expression in Group B were significantly lower than that in Group A (P < 0.001), but they were significantly higher in Groups C, D, E, F, G, and H than those in Group B (P < 0.05).
CONCLUSIONRhubarb monomer treatment ameliorated mucosal damage in sepsis via enhanced transcription, translation, and expression of junction proteins.