A study of mesenchymal stem cell proliferation and surface characteristics of the titanium discs coated with MS275/PLGA by an electrospray.
10.4047/jkap.2012.50.4.285
- Author:
Soo Yeon YOO
1
;
Seong Kyun KIM
;
Seong Joo HEO
;
Jai Young KOAK
;
Joo Hee LEE
;
Yoon Kyung PARK
;
Ena KIM
Author Information
1. Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Korea. ksy0617@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Titanium;
MS275;
PLGA;
Mesenchymal stem cells
- MeSH:
Cell Proliferation;
Cell Survival;
Lactic Acid;
Mesenchymal Stromal Cells;
Polyglycolic Acid;
Titanium
- From:The Journal of Korean Academy of Prosthodontics
2012;50(4):285-291
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was conducted to identify the surface characteristics of titanium discs coated with MS275/PLGA by electrospray and which is effective to mesenchymal stem cell proliferation. MATERIALS AND METHODS: We used anodized surface coated with PLGA as a control group and anodized surface coated with MS275 0.5 microM, 1 microM, 1.5 microM as test groups. To examine that the coating particles are nanometer sized, FE-SEM was used and AFM was utilized to determine the difference of coating surface roughness. We checked the mesenchymal stem cell proliferation by using MTT assay on 1st, 4th, 7th days. RESULTS: There was no significant difference between control groups and test groups in AFM results (P>.05). In MTT assay results, mesenchymal stem cell proliferation was increased with time, at 7th day, cell viability on discs coated with 1.5 microM MS275 was significantly higher than control group (P<.05). As SEM showed, the number of cells on all discs was increased and the morphology of cell attachment was also wider and closer with time. CONCLUSION: Titanium surface coated with MS275/PLGA showed significantly higher cell proliferation and the more density of MS275 was dispersed on titanium discs, the faster cells grew.
