Determination of the content of zotarolimus on the drug eluting stents by HPLC.
- Author:
Yuan LI
1
;
Xi JIANG
;
Bo FAN
;
Hong CUI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drug-Eluting Stents; Sirolimus; analogs & derivatives; analysis
- From: Chinese Journal of Medical Instrumentation 2011;35(2):134-136
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTTo establish a method for the content determination of zotarolimus on the drug eluting stents.
METHODSHPLC analysis was carried out with Zorbax Eclipse C8 column (4.6 x 75 mm 3.5 microm)and MPA acetate buffer solution: acetonitrile (51:49), MPB acetate buffer solution: acetonitrile (5:95) as the mobile phase. The detection wavelength was 278 nm.
RESULTThere was a good linear relationship between the concentration of zotarolimus and the areas in the range of 5 microg/ml-75 microg/ml (r = 0.99986). The average recovery was 101.58% with RSD 0.68% (n = 6).
CONCLUSIONThe method is simple, accurate and reproducible. It can be used as the quality test for zotarolimus on the drug eluting stents.
