Hair roots induction and culture of Withania somnifera and its withanolide A synthesis.
- Author:
Feng-Ying WANG
;
Yi-Ming SUN
;
Cui-Ping LV
;
Meng-Qi CHENG
;
Lai ZHANG
;
Min SUN
- Publication Type:Journal Article
- MeSH:
Agrobacterium tumefaciens;
physiology;
Plant Extracts;
analysis;
biosynthesis;
Plant Roots;
chemistry;
growth & development;
metabolism;
microbiology;
Withania;
chemistry;
growth & development;
metabolism;
microbiology;
Withanolides;
analysis;
metabolism
- From:
China Journal of Chinese Materia Medica
2014;39(5):790-794
- CountryChina
- Language:Chinese
-
Abstract:
Withanolide A is a biologically active secondary metabolite occuring in roots and leaves of Withania somnifera. In the present study, adventitious roots from leaf explants of W. somnifera were induced for the production of withanolide-A by Agrobacterium tumefaciens strain C58C1 to obtain hair roots. Hair roots induction rate reached 30%. The withanolide A was determined by HPLC in different hair roots lines and different parts of W. somnifera. The average content of withanolide A in all hair roots lines were 1.96 times as high as that in wild-plant, the concentration of withanolide A in hair roots (1.783 mg x g(-1) dry weight) were 1.51 times as high as the roots of wild W. somnifera (1.180 mg x g(-1) dry weight), respectively. It is possible to obtain withanolide A from hair roots culture of W. somnifera.
