Study on hydrolysis kinetics of ginsenoside-Ro in alkaline medium and structural analysis of its hydrolyzate.
- Author:
Qiong ZHU
;
De-Kun LI
;
Da-Zheng ZHOU
;
Zheng-Liang YE
- Publication Type:Journal Article
- MeSH:
Drugs, Chinese Herbal;
chemistry;
Ginsenosides;
chemistry;
Hydrogen-Ion Concentration;
Hydrolysis;
Kinetics;
Magnetic Resonance Spectroscopy;
Mass Spectrometry;
Molecular Structure;
Spectrometry, Mass, Electrospray Ionization
- From:
China Journal of Chinese Materia Medica
2014;39(5):867-872
- CountryChina
- Language:Chinese
-
Abstract:
The ginsenoside-Ro is sensitive to be hydrolyzed in an alkaline medium. This paper investigated the hydrolysis kinetics of ginsenoside-Ro under different pH and temperature values. The results showed ginsenoside-Ro in alkaline solution followed pseudo-first-order reaction. Hydrolysis kinetics of ginsenoside-Ro has not been reported previously. The hydrolysis rate was independent of initial concentration. On the basis of UFLC-MS/MS, NMR, as well as chemical evidence,the structure of hydrolyzate was assigned as 3-O- [beta-D-glucuronopyranosyl- (1 --> 2) -beta-D-glucopyranosyl] -oleanolic acid.
