Experimental study on relationship between pungent-hot herb property express and calmodulin.
- Author:
Xin LIU
;
Yan HU
;
Yi-Ran CUI
;
Xiao-Qing LIU
;
Bing-Hua TANG
;
Bing ZHANG
- Publication Type:Journal Article
- MeSH: Animals; Calmodulin; metabolism; Drugs, Chinese Herbal; administration & dosage; chemistry; Gonads; drug effects; metabolism; Hypothalamo-Hypophyseal System; drug effects; metabolism; Liver; drug effects; metabolism; Male; Rats; Rats, Sprague-Dawley; Yang Deficiency; drug therapy; metabolism
- From: China Journal of Chinese Materia Medica 2014;39(5):873-878
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explain the essence of pungent-hot herb property express according to in vivo and in vitro studies on its effect on calmodulin on the base of the observation of the adjustment in hypothalamic-pituitary-gonad axis functions of Aconiti Lateralis Radix Praeparata, Curculiginis Rhizoma, Cinnamomi Cortex and bitter-cold herb Phellodendri Chinensis Cortex in rats under the state of yang deficiency.
METHODThe yang-deficient model was duplicated by intramuscularly injecting hydrocortisone sodium succinate powder injection. After the intervention with Aconiti Lateralis Radix Praeparata, Curculiginis Rhizoma, Cinnamomi Cortex and bitter-cold herb Phellodendri Chinensis Cortex for seven days, TSH, T3, T4, 17-OHCS, COR, T, E2 of hypothalamus-pituitary-target gland axis and other relevant indexes were detected. The calmodulin expression in livers and L02 cells cultured in vitro was detected by using Western blot.
RESULTPungent-hot herbs Aconiti Lateralis Radix Praeparata, Curculiginis Rhizoma, Cinnamomi Cortex can significantly correct indicators of hypothalamic-pituitary-gonad axis and calmodulin, whereas the bitter-cold herb Phellodendri Chinensis Cortex showed no obvious effect.
CONCLUSIONThe pungent-hot herb property expression may be closely related to calmodulin.