Identification of Bupleurum chinense and B. longiradiatum based on ITS2 barcode.
- Author:
Jun-Lin YU
;
Sha ZHAO
;
Ming-Bo REN
;
Qi-Ni QIAN
;
Xiao-Hui PANG
- Publication Type:Journal Article
- MeSH:
Bupleurum;
classification;
genetics;
DNA Barcoding, Taxonomic;
methods;
DNA, Plant;
genetics;
DNA, Ribosomal Spacer;
genetics;
Drugs, Chinese Herbal;
chemistry;
classification;
Molecular Sequence Data;
Phylogeny;
Quality Control
- From:
China Journal of Chinese Materia Medica
2014;39(12):2160-2163
- CountryChina
- Language:Chinese
-
Abstract:
In this study, ITS2 barcode was used to identify Bupleurum chinense and B. longiradiatum. The ITS2 regions of 48 samples were amplified and sequenced. The sequences obtained above were aligned and the K2P distances were calculated. We used three methods, BLAST1, nearest distance and phylogenetic tree (NJ-tree), to test the identification ability. The results showed that the maximum intraspecific genetic distance of B. chinense was 0.013, and the minimum interspecific genetic distance between B. chinense and B. longiradiatum was 0.049. The NJ-tree can easily identify B. chinense and B. longiradiatum. Therefore, the ITS2 barcode is suitable to identify B. chinense and B. longiradiatum.
