Identification of cattail pollen (puhuang), pine pollen (songhuafen) and its adulterants by ITS2 sequence.
- Author:
Xiao-Xi MA
;
Wei SUN
;
Wei-Chao REN
;
Li XIANG
;
Bo ZHAO
;
Ya-Qin ZHANG
;
Ming SONG
;
Ze-Jing MU
;
Shi-Lin CHEN
- Publication Type:Journal Article
- MeSH:
China;
DNA Barcoding, Taxonomic;
methods;
DNA, Plant;
genetics;
DNA, Ribosomal Spacer;
genetics;
Drug Contamination;
prevention & control;
Drugs, Chinese Herbal;
chemistry;
classification;
Molecular Sequence Data;
Phylogeny;
Pinus;
classification;
genetics;
Pollen;
classification;
genetics;
Quality Control;
Typhaceae;
classification;
genetics
- From:
China Journal of Chinese Materia Medica
2014;39(12):2189-2193
- CountryChina
- Language:Chinese
-
Abstract:
DNA barcoding method was conducted for the authentication of pollen materials due to difficulty of discriminating pollen materials bearing morphological similarity. In this study, a specific focus was to identify cattail pollen (Puhuang) and pine pollen (Songhuafen) samples from their adulterants which are frequently mixed-together. Regions of the internal transcribed spacer (ITS2) from 60 samples were sequenced, and new primers for cattail pollen were designed according to the sequence information. The results from the NJ trees showed that the species of pine pollen, Puhuang and their adulterants can be classified as obvious monophyly. Therefore, we propose to adapt DNA barcoding methodology to accurately distinguish cattail pollen, pine pollen and their adulterant materials. It is a great help for drug regulatory agency to supervise the quality of medicinal materials.
