Identification of Placenta hominis and its adulterants using COI barcode.
- Author:
Jun CHEN
;
Jing JIA
;
Xiao-Lan XU
;
Tian-Yi XIN
;
Hong-Yin ZHANG
;
Lin-Chun SHI
;
Hui YAO
;
Dong LIU
;
Zhen-Hong WU
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
DNA Barcoding, Taxonomic;
methods;
Drug Contamination;
prevention & control;
Electron Transport Complex IV;
genetics;
Female;
Humans;
Medicine, Chinese Traditional;
Molecular Sequence Data;
Phylogeny;
Placenta;
chemistry;
enzymology;
Pregnancy;
Quality Control;
Sheep;
Swine
- From:
China Journal of Chinese Materia Medica
2014;39(12):2204-2207
- CountryChina
- Language:Chinese
-
Abstract:
In order to provide a new method for the identification of Placenta hominis, the COI barcode has been employed to identify the P. hominis medicinal materials and its adulterants. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. NJ tree was constructed by MEGA6.0 software. COI sequences can be successfully obtained from all experimental samples. The intra-specific variation and inter-specific divergence were calculated. The average intra-specific K2P distance of P. hominis was 0.001 and the maximum intra-specific distance was 0.008. The cluster dendrogram constructed can be seen that the same genus is together, and distinguished from its adulterants. It is concluded that P. hominis and its adulterants can be correctly identified by DNA barcoding method.
