Identification of Scolopendra subspinipes mutilans and its adulterants using DNA barcode.
- Author:
Hong-Yin ZHANG
;
Jun CHEN
;
Jing JIA
;
Dong LIU
;
Lin-Chun SHI
;
Hui ZHANG
;
Jing-Yuan SONG
;
Hui YAO
- Publication Type:Journal Article
- MeSH:
Animals;
Arthropod Proteins;
genetics;
DNA Barcoding, Taxonomic;
methods;
Drug Contamination;
prevention & control;
Electron Transport Complex IV;
genetics;
Medicine, Chinese Traditional;
Molecular Sequence Data;
Phylogeny;
Quality Control;
Scorpions;
classification;
enzymology;
genetics
- From:
China Journal of Chinese Materia Medica
2014;39(12):2208-2211
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the COI barcode was used to identify the Scolopendra medicinal materials and its adulterants in order to provide a new method for the identification of Scolopendra. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence alignment and NJ tree construction was carried out by MEGA6.0 software. The results showed that the COI sequences can be obtained from all experimental samples. The average inter-specific K2P distance of Scolopendra was 0.222 and the minimum inter-specific distance was 0.190. All the Scolopendra subspinipes mutilans medicinal samples clustered into a clade in the NJ tree and can be distinguished from its adulterants. In a conclusion, COI can be used to correctly identify Scolopendra medicinal materials, and it will be a potential DNA barcode for identifying other animal medicinal materials.
