Molecular identification of Manis pentadactyla using DNA barcoding.
- Author:
Jing JIA
;
Hong-Yin ZHANG
;
Jun CHEN
;
Dong LIU
;
Hui YAO
;
Qi-Ni QIAN
;
Hui ZHANG
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
DNA Barcoding, Taxonomic;
methods;
Drug Contamination;
prevention & control;
Electron Transport Complex IV;
genetics;
Mammals;
classification;
genetics;
Medicine, Chinese Traditional;
Molecular Sequence Data;
Phylogeny;
Quality Control;
Sheep;
Swine
- From:
China Journal of Chinese Materia Medica
2014;39(12):2212-2215
- CountryChina
- Language:Chinese
-
Abstract:
The COI gene as DNA barcode was used to identify the Manis pentadactyla and its adulterants in order to provide a scientific basis for the molecular identification of M. pentadactyla. Genomic DNA was extracted from experimental samples using the DNA extraction kit. The COI genes were amplified using polymerase chain reaction (PCR) and sequenced bi-directionally. Obtained sequences were assembled using the CodonCode Aligner. The neighbor-joining (NJ) tree was constructed by MEGA 6.0. The results indicated that COI sequences were successfully amplified and NJ trees results indicated that M. pentadactyla and its adulterants can be easily identification. Therefore, the COI gene is an efficient barcode for identification of M. pentadactyla and its adulterants,which will provide a new technique for the market supervision.
