Identification of pyrrosiae folium and its adulterants based on psbA-trnH sequence.
- Author:
Ya-Qin ZHANG
;
Yue SHI
;
Ming SONG
;
Yun-Han LIN
;
Xiao-Xi MA
;
Wei SUN
;
Li XIANG
;
Xi LIU
- Publication Type:Journal Article
- MeSH:
Base Sequence;
DNA Barcoding, Taxonomic;
methods;
DNA, Ribosomal Spacer;
genetics;
Drug Contamination;
prevention & control;
Drugs, Chinese Herbal;
chemistry;
classification;
Ferns;
classification;
genetics;
Molecular Sequence Data;
Phylogeny;
Plant Proteins;
genetics;
Quality Control
- From:
China Journal of Chinese Materia Medica
2014;39(12):2222-2226
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the psbA-trnH sequence as DNA barcode was used to evaluate the accuracy and stability for identification pteridophyte medicinal material Pyrrosiae Foliumas from adulterants. Genomic DNA from 106 samples were extracted successfully. The Kimura 2-Parameter (K2P) distances and ML tree were calculated using software MEGA 6.0. The intra-specific genetic distances of 3 original plants were lower than inter-specific genetic distances of adulterants. The ML tree indicated that Pyrrosiae Folium can be distinguished from its adulterants obviously. Therefore, the psbA-trnH sequence as a barcode of the pteridophyte, can accurately and stably distinguish Pyrrosiae Folium from its adulterants.
