In silico cloning and bioinformatics analysis of an AP2/EFR family gene from Arnebia euchroma.
- Author:
Teng XIE
;
Sheng WANG
;
Liang-Yun ZHOU
;
Jin-Fu TANG
;
Lan-Ping GUO
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Base Sequence;
Boraginaceae;
classification;
genetics;
Cloning, Molecular;
Computational Biology;
Computer Simulation;
Hydrophobic and Hydrophilic Interactions;
Molecular Sequence Data;
Phylogeny;
Plant Proteins;
genetics;
Protein Structure, Secondary;
Protein Structure, Tertiary;
Transcription Factors;
chemistry;
genetics
- From:
China Journal of Chinese Materia Medica
2014;39(12):2251-2257
- CountryChina
- Language:Chinese
-
Abstract:
A cDNA sequence of Arnebia euchroma AP2/ERF named AeAP2/ERF was cloned by in silico cloning in this study, using ACX71873 sequence from Lithospermum erythrorhizon as the probe sequence. Some characters of the AP2/ERF gene and encoded protein sequences were predicted and analyzed by the bioinformatics methods, including general physical and chemical properties, hydrophobieity, signal peptide, secondary structure, localization sites in cells. Results showed that the 876 bp long gene included a 1 077 bp ORF and encoding 205 amino acid. The AeAP2/ERF protein had no signal peptide, it was a hydrophilic proteins located in nucleus. The function of the AP2/ERF protein was mainly involved with metabolism controlling and signal transduction.
