Synthesis and identification of artificial antigen of forsythin.
- Author:
Ya-Jun REN
;
Hui-Hua QU
;
Jin-Jun CHENG
;
Na-Na HE
;
Sheng-Lan FENG
;
Ling-Ling ZHAO
;
Yan ZHAO
;
Qing-Guo WANG
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies;
immunology;
Antigens;
chemistry;
immunology;
Bridged Bicyclo Compounds, Heterocyclic;
chemistry;
immunology;
Drugs, Chinese Herbal;
chemistry;
Furans;
chemistry;
immunology;
Male;
Mice;
Mice, Inbred BALB C
- From:
China Journal of Chinese Materia Medica
2014;39(12):2295-2299
- CountryChina
- Language:Chinese
-
Abstract:
The establishment of high specificity and sensitivity method of small molecule monoclonal antibody-based immunoassay has a great importance in the study of small molecule compounds in Chinese medicine, wherein synthesis of small molecule artificial antigen is a critical step in the preparation of small molecule antibodies. Oxidation method using sodium iodide was used to synthesize immunogenic antigen (FRn-BSA) and coating antigen (FRn-OVA) of forsythin. UV spectroscopy and thin layer chromatography showed that forsythin was successfully conjugated with BSA and OVA. After immuned FRn-BSA, the mice could specifically produce anti-forsythin antibodies with titer up to 1:8 000, and the linear range was from 1 mg x L(-1) to 100 mg x L(-1). In this paper, the artificial antigen of forsythin was successfully synthesized, which can be applied for preparation of monoclonal antibodies and establishment of appropriate immune method.
