Intervention effect of quercetin on inflammatory secretion of cardiac fibroblasts.
- Author:
Xi-Lan TANG
;
Jian-Xun LIU
;
Wei DONG
;
Peng LI
;
Lei LI
;
Yong-Qiu ZHENG
;
Jin-Cai HOU
- Publication Type:Journal Article
- MeSH:
Animals;
Cells, Cultured;
Drugs, Chinese Herbal;
administration & dosage;
Endomyocardial Fibrosis;
drug therapy;
genetics;
immunology;
Female;
Fibroblasts;
drug effects;
immunology;
Heart;
drug effects;
Humans;
Interleukin-6;
genetics;
immunology;
Male;
Proto-Oncogene Proteins c-akt;
genetics;
immunology;
Quercetin;
administration & dosage;
Rats;
Rats, Sprague-Dawley;
Tumor Necrosis Factor-alpha;
genetics;
immunology
- From:
China Journal of Chinese Materia Medica
2014;39(12):2314-2317
- CountryChina
- Language:Chinese
-
Abstract:
To establish neonatal rat cardiac fibroblast inflammatory secretion model by using LPS 100 microg x L(-1) combined with ATP 5 mmol x L(-1), in order to study the inhibitory effect of quercetin on the secretion of inflammatory factors TNF-alpha, IL-1beta and IL-6 of cardiac fibroblasts, further investigate the effect of quercetin on the protein expression of p-NF-kappaB p65 (S276) and p-Akt (S473) by western blot, and discuss the inhibitory effect of quercetin on the inflammatory secretion of cardiac fibroblasts. According to the findings, quercetin with the concentrations between 51.74 micromol x L(-1) and 827.81 micromol x L(-1) had no significant effect on the activity of cardiac fibroblasts. Quercetin with the concentrations of 82.78, 41.39, 20.70 micromol x L(-1) could notably inhibit the increase of TNF-alpha and IL-1beta induced by LPS 100 microg x L(-1) for 3 h and then ATP 5 mmol x L(-1) for 36 h (P < 0.01 or P < 0.05). Quercetin with the concentrations of 82.78, 41.39 micromol x L(-1) could notably inhibit the increase of IL-6 induced LPS 100 microg x L(-1) for 3 h and then ATP 5 mmol x L(-1) for 36 h (P < 0.05), without any notable effect of quercetin with the concentration of 20.70 micromol x L(-1). Quercetin with the concentrations of 82.78, 41.39, 20. 70 micromol x L(-1) could notably inhibit the NF-kappaB p65 (S276) activation induced by LPS 100 microg x L(-1) for 3 h and then ATP 5 mmol x L(-1) for 15 min, with the most significant effect in 20.70 micromol x L(-1). Quercetin with the concentrations of 82.78, 41.39, 20.70 micromol x L(-1) could notably inhibit the increase of p-Akt(473) expression induced by LPS 100 microg x L(-1) for 3 h and then ATP 5 mmol x L(-1) for 240 min (P < 0.05). Therefore, this study believes that quercetin could attenuate the secretion of inflammatory factors TNF-alpha, IL-1beta and IL-6 of cardiac fibroblasts by inhibiting the activation of NF-kappaB p65 (S276) and Akt (473).
