UPLC-MS/MS determination of content of three iridoids of xingnaojing oral preparation in rat brains and study on their brain pharmacokinetics.
- Author:
Pan XU
;
Shou-Ying DU
;
Yang LU
;
Jie BAI
;
Hui-Min LIU
;
Qiu DU
;
Zhen-Zhen CHEN
;
Zhen WANG
- Publication Type:Journal Article
- MeSH:
Animals;
Brain;
metabolism;
Brain Chemistry;
Chromatography, High Pressure Liquid;
methods;
Drug Stability;
Drugs, Chinese Herbal;
chemistry;
pharmacokinetics;
Iridoids;
chemistry;
pharmacokinetics;
Male;
Rats;
Rats, Sprague-Dawley;
Tandem Mass Spectrometry;
methods
- From:
China Journal of Chinese Materia Medica
2014;39(12):2351-2355
- CountryChina
- Language:Chinese
-
Abstract:
To establish a UPLC-MS/MS method for the simultaneous determination of geniposide, genipin 1-O-beta-D-gentiobioside and geniposidic acid in rat brains and study the brain pharmacokinetics of the three iridoid glycosides in stroke rat after the oral administration of Xingnaojing. In this experiment, brain samples were precipitated with protein for twice. Acquity BEH C18 column was adopted, with acetonitrile-0.1% formic acid-water as the mobile phase for gradient elution. ESI source was adopted for mass spectra; multiple reaction monitoring (MRM) was conducted to detect negative ions. The time for sample analysis was 3.5 min. the results showed good linear relations among the three iridoid glycosides, with the extraction recovery between 99.6% and 114.3%, good intra- and inter-day precisions and accuracies and stability in line with the requirements. The t1/2 and MRT in the three components were similar in brains of stroke rats. Geniposide and genipin 1-O-beta-D-gentiobioside showed double peaks; where as geniposidic acid showed a single peak. In conclusion, the method is so specific, sensitive, accurate and reliable that it can be used to study the brain pharmacokinetics of Xingnaojing oral preparation.
