Optimization of synthetic pathway and fermentation process of yeast cell factories for production of oleanoic acid.
- Author:
Dong WANG
;
Bei-Bei WANG
;
Yi LIU
;
Ming-Yu SHI
;
Dong-Guang XIAO
;
Lu-Qi HUANG
;
Zhu-Bo DAI
;
Xue-Li ZHANG
- Publication Type:Journal Article
- MeSH: Biomass; Biotechnology; methods; Dose-Response Relationship, Drug; Fermentation; Glucose; pharmacology; Oleanolic Acid; biosynthesis; Saccharomyces cerevisiae; cytology; drug effects; metabolism
- From: China Journal of Chinese Materia Medica 2014;39(14):2640-2645
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the synthetic pathway and fermentation process of yeast cell factories for production of oleanoic acid.
METHODUsing the DNA assembler method, one copy of Glycyrrhiza glabra beta-amyrin synthase (GgbAS), Medicago truncatula oleanolic acid synthase (MtOAS) and Arabidopsis thaliana cytochrome P450 reductase 1 (AtCPR1) genes were introduced into Saccharomyces cerevisiae strain BY-OA, resulting in strain BY-20A. YPD medium with different glucose concentration were then used to cultivate strain BY-2OA.
RESULTIncreasing gene copies of GgbAS, MtOAS and AtCPR1 resulted in increased beta-amyrin and oleanolic acid production. The strain BY-2OA produced 136.5 mg x L(-1) beta-amyrin and 92.5 mg x L(-1) oleanolic acid, which were 54% and 30% higher than the parent strain BY-OA. Finally, the titer of oleanolic acid increased to 165.7 mg x L(-1) when cultivated in YPD medium with 40 mg x L(-1) glucose.
CONCLUSIONProduction of oleanoic acid increased significantly in the yeast strain BY-2OA, which can provide the basis for creating an alternative way for production of oleanoic acid in place of extraction from plant sources.