UPLC fingerprint spectra for discrimination of Aucklandiae radix and Vladimiriae radix.
- Author:
Lu-Yang LV
;
Ji-Zhong ZHANG
;
Zhi-Feng ZHANG
;
Yuan LIU
;
Rui ZENG
;
Jian-Mei LU
;
Huan-Ming REN
- Publication Type:Journal Article
- MeSH:
Asteraceae;
chemistry;
classification;
Chromatography, High Pressure Liquid;
Cluster Analysis;
Drugs, Chinese Herbal;
analysis;
chemistry
- From:
China Journal of Chinese Materia Medica
2014;39(14):2699-2703
- CountryChina
- Language:Chinese
-
Abstract:
It's difficult to identify Aucklandiae Radix and Vladimiriae Radix because of their similar composition. In this paper, UPLC method was used to establish their UPLC fingerprint to identify them with the mobile of acetonitrile -0. 05% phosphoric acid water solution by gradient elution at the detection wavelength of 238 nm. Clustering analysis and principal components analysis showed that Vladimiriae Radix was significantly different from Aucklandiae Radix. Eight common peaks and twelve common peaks were defined respectively in Aucklandiae Radix and Vladimiriae Radix herbs by fingerprint analysis. Six of them were identified as syringoside, chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, costunolide and dehydrocostuslactone by comparing with standard references. There are four peaks in all of Vladimiriae Radix samples and in none of Aucklandiae Radix samples. So UPLC fingerprint can be used to identify these two herbs.
