Researching on fingerprint of Inulacappa by HPLC.
- Author:
Di-fei-fei XIONG
;
Di ZHU
;
Dan TAN
;
Yan-yu LAN
;
Lin ZHENG
;
Ai-min WANG
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Inula; chemistry
- From: China Journal of Chinese Materia Medica 2015;40(3):480-483
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study is to establish the fingerprint and find out the common chromatographic peaks of Inula cappa by HPLC.
METHODThe HPLC analysis was performed on an Agilent Eclipse Plus C18 column (2.1 mm x 150 mm, 1.8 μm) with 0.1% fomic acid aqueous solution-0.1% fomic acid acetonitrile solution as mobile phase at a flow rate of 0.3 · mL(-1) · min(-1); The detective wavelength is 325 nm; The column temperature is 45 °C.
RESULTThe results indicated that 5 of 17 common peaks were identified . The similarity about 10 groups of Inulacappais is over 0.95.
CONCLUSIONThis method is able to be a scientific basis of quality assessment according to its convenient and reliable.