Study on identification of "Digeda" raw materials in Mongolian patent medicine by PCR amplification of specific alleles.
- Author:
Zhan-hu CUI
;
Xian-zhang HUANG
;
Ping LONG
;
Le ZHANG
;
Dong-dong ZHAO
;
Ying-li WANG
;
Min-hui LI
- Publication Type:Journal Article
- MeSH:
Alleles;
DNA Primers;
genetics;
DNA, Plant;
genetics;
Medicine, Mongolian Traditional;
Molecular Sequence Data;
Plants, Medicinal;
classification;
genetics;
Polymerase Chain Reaction;
methods
- From:
China Journal of Chinese Materia Medica
2015;40(5):793-798
- CountryChina
- Language:Chinese
-
Abstract:
To explore a new method for identification of Mongolian patent medicine (MPM) by PCR amplification of specific alleles. Eight kinds of MPM were used to study the identification of "Digeda" raw materials. The total DNA of Lomatogonium rotatum and Corydalis bungeana samples were extracted through modified CTAB method, psbA-trnH sequence was amplified by PCR and sequenced directionally. Specific primer was designed. The DNA of 8 kinds of MPM also was extracted and purified by the commercial DNA purification kits. The rbcL and two pair of specific primers sequences were amplified. The specific amplified products were sequenced in forward directions. All specific sequences were aligned and were analyzed. The results indicated that L rotatum can be identified by specific primers from Digeda-4 Tang, Digeda-8 San, Digeda-4 San, and C. bungeana medicinal materials can be identified by specific primers from Li Dan Ba Wei San, Yi He Ha Ri-12 and A Ga Ri-35. PCR amplification of specific alleles can stably and accurately distinguish raw medicinal materials in MPM.
