Cloning and expression analysis of a acetyl-CoA U-acetyltransferase gene (TwAACT) from Tripterygium wilfordii.
- Author:
Yu-jun ZHAO
;
Meng ZHANG
;
Yu-jia LIU
;
Ping SU
;
Tian-yuan HU
;
Xin CHEN
;
Wei GAO
;
Lu-qi HUANG
- Publication Type:Journal Article
- MeSH:
Acetyl-CoA C-Acetyltransferase;
chemistry;
genetics;
metabolism;
Amino Acid Sequence;
Cloning, Molecular;
Gene Expression Regulation, Plant;
Models, Molecular;
Molecular Sequence Data;
Phylogeny;
Plant Proteins;
chemistry;
genetics;
metabolism;
Sequence Alignment;
Tripterygium;
chemistry;
classification;
enzymology;
genetics
- From:
China Journal of Chinese Materia Medica
2015;40(5):847-852
- CountryChina
- Language:Chinese
-
Abstract:
In this study, based on the transcriptome data, we cloned the full-length cDNAs of TwAACT gene from Tripterygium wilfordii suspension cells, and then analyzed the bioinformation of the sequence, detected the genetic differential expression after being induced by methyl jasmonate (MeJA) by RT-PCR. The full-length cDNA of the TwAACT was 1 704 bp containing a 1 218 bp open reading frame (ORF) encoding a polypeptide of 405 amino acids (GeneBank accession No. KP297934). The deduced isoelectric point (pI) was 6.10, a calculated molecular weight was about 41.20 kDa, and online prediction showed that TwAACT had two catalytic active sites. After the induction of MeJA, the relative expression level of TwAACT increased rapidly. The expression level of TwAACT was highest at 24 h. TwAACT was cloned firstly, that laid the foundation for identifying thegene and illustrating thebiosynthesis mechanism and its synthetic biology.
