Gene cloning and bioinformatics analysis of new gene for chlorogenic acid biosynthesis of Lonicera hypoglauca.
- Author:
Shu-lin YU
;
Lu-qi HUANG
;
Yuan YUAN
;
Lin-jie QI
;
Da-hui LIU
- Publication Type:Journal Article
- MeSH:
Chlorogenic Acid;
metabolism;
Cloning, Molecular;
Computational Biology;
Gene Expression Regulation, Plant;
Lonicera;
chemistry;
classification;
genetics;
metabolism;
Molecular Sequence Data;
Phylogeny;
Plant Proteins;
chemistry;
genetics;
metabolism;
Protein Structure, Secondary
- From:
China Journal of Chinese Materia Medica
2015;40(5):863-867
- CountryChina
- Language:Chinese
-
Abstract:
To obtain the key genes for chlorogenic acid biosynthesis of Lonicera hypoglauca, four new genes ware obtained from the our dataset of L. hypoglauca. And we also predicted the structure and function of LHPAL4, LHHCT1 , LHHCT2 and LHHCT3 proteins. The phylogenetic tree showed that LHPAL4 was closely related with LHPAL1, LHHCT1 was closely related with LHHCT3, LHHCT2 clustered into a single group. By Real-time PCR to detect the gene expressed level in different organs of L. hypoglauca, we found that the transcripted level of LHPAL4, LHHCT1 and LHHCT3 was the highest in defeat flowers, and the transcripted level of LHHCT2 was the highest in leaves. These result provided a basis to further analysis the mechanism of active ingredients in different organs, as well as the element for in vitro biosynthesis of active ingredients.
