Chemical constituents of Swertia delavayi and their anti-hepatitis B virus activity.
- Author:
Tuan-wu CAO
;
Chang-an GENG
;
Yun-bao MA
;
Kang HE
;
Ning-jia ZHOU
;
Jun ZHOU
;
Xue-mei ZHANG
;
Ji-jun CHEN
- Publication Type:Journal Article
- MeSH:
Antiviral Agents;
chemistry;
isolation & purification;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
Hepatitis B virus;
drug effects;
genetics;
Magnetic Resonance Imaging;
Molecular Structure;
Spectrometry, Mass, Electrospray Ionization;
Swertia;
chemistry
- From:
China Journal of Chinese Materia Medica
2015;40(5):897-902
- CountryChina
- Language:English
-
Abstract:
Fifteen known compounds were isolated from Swertia delavayi by silica gel, Sephadex LH-20 and Rp-18 column chromatographies. Based on extensive spectroscopic analysis (MS, 1H, 13C-NMR), their structures were identified aserythrocentaurin (1), erythrocentaurindimethylacetal (2), sweroside (3), swertiamarin (4), gentiopicroside (5), swertiakoside A (6), 2'-O-acetylswertiamarin (7), 4'-O-[(Z) -coumaroyl] swertiamarin (8), 1,5,8-trihydroxy-3-methoxyxanthone (9), 8-O-β-D-glucopyranosyl-1-hydroxy-2,3, 5-trimethoxyxanthone (10), 8-O-[β-D-xyl- opyranosyl-(1 --> 6)-β-D-glucopyranosyl]-7,8-dihydroxy-3-methoxyxanthone (11), isovitexin (12), β-sitosterol (13), daucosterol (14), and oleanolic acid (15). Among them, ten ones (14, 7-11, 13) were obtained from S. delavayi for the first time. The isolates were evaluated for their anti-HBV activities in HepG 2. 2. 15 cell line in vitro. The results showed that compound 1, 2, 6, 7, 9 and 12 exhibited significant inhibitory activity on HBV DNA replication with IC50 values from 0.05 to 1.46 mmol x L(-1).
