Effect of Siwu decoction on function and expression of P-glycoprotein in Caco-2 cells.
- Author:
Yi JIANG
;
Zeng-chun MA
;
Xian-ju HUANG
;
Qing YOU
;
Hong-ling TAN
;
Yu-guang WANG
;
Qian-de LIANG
;
Xiang-lin TANG
;
Cheng-rong XIAO
;
Yue GAO
- Publication Type:Journal Article
- MeSH:
ATP Binding Cassette Transporter, Sub-Family B;
genetics;
metabolism;
ATP-Binding Cassette, Sub-Family B, Member 1;
genetics;
metabolism;
Caco-2 Cells;
Drugs, Chinese Herbal;
pharmacology;
Humans;
Up-Regulation;
drug effects
- From:
China Journal of Chinese Materia Medica
2015;40(5):933-937
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of Siwu decoction on the function and expression of P-glycoprotein (P-gp) in Caco-2 cells. The Real-time quantitative poly-merase chain reaction (Q-PCR) was used to analyze the mRNA expression of MDR1 gene in Caco-2 cells. Flow cytometer was used to study the effect of Siwu decoction on the uptake of Rhodamine 123 in Caco-2 cells, in order to evaluate the efflux function of P-gp. Western blotting method was used to detect the effect of Siwu decoction on the P-gp protein expression of Caco-2 cells. Compared with the blank control group, after Caco-2 incubation with Siwu decoction at concentrations of 3.3, 5.0, 10.0 g x L(-1) for 24, 48, 72 h, the mRNA expression of MDR1 was up-regulated, suggesting the effect of Siwu decoction in inducing the expression of MDR1. After the administration with Siwu decoction in Caco-2 cells for 48 h, the uptake of Rhodamine 123 in Caco-2 cells decreased by respectively 16.6%, 22.1% (P < 0.05) and 45.4% (P < 0.01), indicating that the long-term administration of Siwu decoction can enhance the P-gp efflux function of Caco-2 cells. After the incubation of Caco-2 cells with Siwu decoction for 48 h, the P-gp protein expression on Caco-2 cell emebranes, demonstrating the effect of Siwu decoction in inducing the protein expression of P-gp.
