Effect of shikonin on proliferation of keratinocytes induced by interleukin-17 and expression of chemokines.
- Author:
Xin-ran XIE
;
Lei ZHANG
;
Xin LIU
;
Yan LIN
;
Zhang LU
;
Ping LI
- Publication Type:Journal Article
- MeSH: Cell Line; Cell Proliferation; drug effects; Chemokines; genetics; metabolism; Drugs, Chinese Herbal; pharmacology; Humans; Interleukin-17; genetics; metabolism; Keratinocytes; cytology; drug effects; metabolism; Naphthoquinones; pharmacology
- From: China Journal of Chinese Materia Medica 2015;40(5):946-949
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of shikonin on the proliferation of human keratinocytes induced by IL-17 and secretion of chemokines, in order to discuss the mechanism of Shikonin in the treatment of psoriasis.
METHODIn vitro cultured HaCaT cells were stimulated by IL-17A (200 μg x L(-1)) and mixed with different concentrations (2, 1 mg x L(-1)) of shikonin for 24 hours. The cell proliferation was detected by CCK-8 assay. Cell secretion inflammatory factor interleukin-23 (IL-23) was detected by ELISA. The expressions of intracellular chemokines CXCL1, CXCL2, CCL20 and 6-defensin 4 (DEFB4) were detected by Real-time PCR.
RESULTShikonin (2,1 mg x L(-1)) could distinctly inhibit HaCaT cell proliferation induced by IL-17A, with statistical difference (P < 0.01). Each shikonin group showed decreases in the secretion of IL-23 and inhibition in expressions of intracellular CXCL1, CXCL2, CCL20 and DEFB4.
CONCLUSIONShikonin could inhibit HaCaT cells proliferation induced by IL-17 and secretion of relevant cytokines and recruit leukocytes by inhibiting chemokines, so as to show the effect in treating psoriasis.