Isolation and antimicrobial activities of actinomycetes from vermicompost.
- Author:
Xue-jun WANG
;
Shuang-lin YAN
;
Chang-li MIN
;
Yan YANG
- Publication Type:Journal Article
- MeSH:
Actinobacteria;
chemistry;
classification;
genetics;
isolation & purification;
Animals;
Anti-Bacterial Agents;
isolation & purification;
metabolism;
Bacteria;
drug effects;
Feces;
microbiology;
Molecular Sequence Data;
Oligochaeta;
Phylogeny;
Quality Control
- From:
China Journal of Chinese Materia Medica
2015;40(4):614-618
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, actinomycetes were isolated from vermicompost by tablet coating method. Antimicrobial activities of actinomycetes were measured by the agar block method. Strains with high activity were identified based on morphology and biochemical characteristics, as well as 16S rDNA gene sequence analysis. The results showed that 26 strains of actinomycetes were isolated, 16 of them had antimicrobial activities to the test strains which accounts for 61.54% of all strains. Among the 16 strains, the strain QYF12 and QYF22 had higher antimicrobial activity to Micrococcus luteus, with a formed inhibition zone of 27 mm and 31 mm, respectively. While the strain QYF26 had higher antimicrobial activity to Bacillus subtilis, and the inhibition zone diameter was 21 mm. Based on the identification of strains with high activity, the strain QYF12 was identified as Streptomyces chartreusis, the strain QYF22 was S. ossamyceticus and the strain QYF26 was S. gancidicus. This study provided a theoretical basis for further separate antibacterial product used for biological control.
