Clearance of Amphotericin B in Rabbit Vitreous.
- Author:
Moo Hyun KIM
1
;
Byung Guk PAK
;
Jang Hyun CHUNG
;
Sang Ha KIM
;
Sung Yong SEOL
Author Information
1. Department of Ophthalmology, School of Medicine, Busan National University, Korea.
- Publication Type:In Vitro ; Original Article
- MeSH:
Amphotericin B*;
Animals;
Candida;
Diffusion;
Half-Life;
Humans;
Indicator Dilution Techniques;
Intravitreal Injections
- From:Journal of the Korean Ophthalmological Society
1982;23(3):547-554
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The object of this paper is to determine the clearance of amphotericin B from the rabbit vitreous. In vitro susceptability of Candida guilliermondi to amphotericin B in antibiotic medium 20(Difco) provided the lowest values of the minimal inhibitory concentration(MIC) among several Candida strains and showed the same MIC afteir incubation at either 30 degrees C or 35 degrees C for 48 hours. A volume of 0.05 ml of solution containing 5 microgram of amphotericin B was injected into the rabbit vitreous, and animals were killed at various intervals. Both eyes were immediately enucleated and frozen to prevent further diffusion of the amphotericin B out of the vitreous. The vitreous was removed intact while it was still frozen. After the vitreous had thawed, its volume was measured. Sterile saline was added and total volume was adjust to 2 ml. The mixture was homogenized and then centrifuged at 2,000 rpm for 20 minutes. One ml of supernatant was removed from the sediment, and the concentration of amphotericin B was determined by broth dilution technique with Candida guilliermondi. The clearance studies indicated that 5 microgram of amphotericin B injected intravititreally, maintained an antibiotic level of greater than 1 microgram/ml for longer than 96 hours, with a half-life of 12 hours. If all factors are considered equal, presumed clearance data for intravitreal injection of 10 microgram of amphotericin B in humans may be calculated from the result in rabbit vitreous. Therapeutic levels of amphotericin B(1 microgram/ml) may be maintained at least 48 hours after the initial intravitreal injection of antibiotic in human eyes.
