Full-length cDNA cloning of flavonol synthase genes of Carthamus tinctorius and construction plant expression vector.
- Author:
Wen-ting YANG
;
Xiu-ming LIU
;
Qiu WAN
;
Na YAO
;
Nan WANG
;
Xue-meng ZHANG
;
Zhong-da JIAO
;
Hai-yan LI
;
Xiao-kun LI
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Base Sequence;
Carthamus tinctorius;
classification;
enzymology;
genetics;
Cloning, Molecular;
DNA, Complementary;
genetics;
metabolism;
Molecular Sequence Data;
Open Reading Frames;
Oxidoreductases;
genetics;
metabolism;
Phylogeny;
Plant Proteins;
genetics;
metabolism
- From:
China Journal of Chinese Materia Medica
2015;40(4):634-638
- CountryChina
- Language:Chinese
-
Abstract:
Flavonol synthase (FLS) is one of the key enzymes in flavonoids metabolic pathways. In this study, middle sequence was obtained from Carthamus tinctorius transcriptome sequencing results. Full-length cDNAs of FLS was cloned from petals of C. tinctorius to FLS by using RT-PCR and RACE technology. Its full-length cDNA was 1,201 bp, with an open reading frame of 1,101 bp and 336 encoded amino acids. The phylogenetic analysis showed that, FLS gene encoded amino acids in C. tinctorius were highly homologous with amino acids in congeneric Compositae species, especially Rudbeckia laciniata. The pBASTA-FLS plant expression vector was successfully built by the molecular biology method, which lays a foundation for further studying biology functions of the gene and biosynthesis mechanism of flavonoids.
