Simultaneous determination of four flavonoids in Wikstroemia indica by HPLC.
- Author:
Li-xia SUN
;
Li-xin SUN
;
Shan-xue MU
;
Lan WEI
;
Cui-ling XU
;
Ming-jing JIA
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
analysis;
Flavonoids;
analysis;
Wikstroemia;
chemistry
- From:
China Journal of Chinese Materia Medica
2015;40(4):700-703
- CountryChina
- Language:Chinese
-
Abstract:
The HPLC method was established to simultaneously determine the contents of myricetin, luteolin, apigenin and kaempferol in Wikstroemia indica ( L. ) C. A. Mey. The method was carried out on a Diamonsil C18 column (4. 6 mm x 250 mm, 5 µm) eluted with the mobile phases of water containing 0.15% phosphoric acid and acetonitrile in gradient mode. The UV detection wavelength was 365 nm. The flow rate was 1.0 mL · min(-1) and the column temperature was set at 30 °C. All the standard compounds showed a good linearity in the range of 0.100 8-1.008 (r = 0.999 2), 0.484 8-4.848 (r = 0.999 0) , 1. 354-13. 54 (r = 0.999 6), 0.316 8-3.168 mg · L(-1) (r = 0.999 0) for myricetin, luteolin, apigenin and kaempferol, respectively. The average recoveries of these four flavonoids were 98.5%, 100.9%, 99.7% and 98.9% with RSD 1.2%, 1.7%, 0.81% and 1.6%, respectively. In conclusion, the method is simple, rapid and accurate. It can be applied for the quality control of Wikstroemia indica.
