Contamination level of aflatoxin B1 in lotus seeds rapid screening by indirect competitive ELISA method.
- Author:
Xian-feng CHU
;
Xiao-wen DOU
;
Wei-jun KONG
;
Mei-hua YANG
;
Chong ZHAO
;
Ming ZHAO
;
Zhen OUYANG
- Publication Type:Journal Article
- MeSH:
Aflatoxin B1;
analysis;
Drug Contamination;
Enzyme-Linked Immunosorbent Assay;
methods;
Loteae;
chemistry;
Seeds;
chemistry
- From:
China Journal of Chinese Materia Medica
2015;40(4):704-709
- CountryChina
- Language:Chinese
-
Abstract:
A simple and cost-effective indirect competitive enzyme-linked immune sorbent assay (ic-ELISA) was developed to rapidly screen the content of aflatoxin B1 (AFB1) in lotus seeds, and the results were confirmed by ultra-fast liquid chromatography-tandem mass spectrometry( UFLC-MS/MS). Matrix-matched calibration expressed a good linearity ranging from 0. 171 to 7. 25 µg · L(-1) for AFB, with R2 > 0.978. The medium inhibitory concentration( IC50 ) for AFB1 was 1.29 µg · L(-1), the recovery for AFB1 was 74.73% to 126.9% with RSD < 5%, and the limit of detection (IC10) was 0.128 µg · L(-1). The developed ic-ELSIA method was applied to rapid analysis of AFB, in 20 lotus seeds samples and the results indicated that the contents of AFB, in samples 1-15 were in the range of 1. 19- 115. 3 µg · kg(-1) and in 40% of the samples exceeded the legal limit(5 µg · kg(-1)), while the contamination rate of AFB, in samples 16-20 was 40%. Pearson correlation coefficient(r) reached 0.997 for AFB1 content in the samples detected by ic-ELSIA and UFLC-MS/MS methods. The results proved that the developed ic-ELISA method is simple, sensitive and reliable, and can be used for rapid and high-throughput screening of AFB1 in lotus seeds
