Changes of ion channel in rat hippocampal neurons during primary culture.
- Author:
Sheng YANG
1
;
Zhen-wei LIU
;
Qin WAN
;
Wen-xia ZHOU
;
Yong-xiang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Calcium; metabolism; Calcium Channels, L-Type; metabolism; Cell Membrane; metabolism; Cells, Cultured; Cellular Senescence; Hippocampus; cytology; Ion Channels; metabolism; Neurons; metabolism; Patch-Clamp Techniques; Rats; Rats, Wistar; Receptors, N-Methyl-D-Aspartate; metabolism; Time Factors
- From: Chinese Journal of Applied Physiology 2004;20(2):151-155
- CountryChina
- Language:Chinese
-
Abstract:
AIM AND METHODSWhole-cell recording technique was used to observe the changes of voltage-dependent ion channels and NMDA receptor currents of rat hippocampal neurons during primary culture.
RESULTSThere was no significant difference of voltage-dependent Na+ current (I(Na)) at 7 d, 14 d and 21 d in culture. It's the same for delayed rectifier K+ current (Ik). However, voltage-dependent Ca2+ current (I(Ca)) and its density were continuously and markedly increased. Further studies showed that the increase of I(Ca) was resulted from the increase of L-type voltage-dependent Ca2+ channels (L-VDCC). NMDA receptor current was also significantly increased with time of culture.
CONCLUSIONCa2+ influx through VDCC and NMIDA receptor is the fatal factor in the aging and death of hippocampal neurons.
