Protective effects of adenovirus-mediated hepatocyte growth factor on injury of rat cortex neurons.
- Author:
Yun HUANG
1
;
Xiao-qin HA
;
Chu-ze WU
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Animals, Newborn; Cells, Cultured; Cerebral Cortex; drug effects; Culture Media, Serum-Free; Hepatocyte Growth Factor; genetics; pharmacology; Neurons; drug effects; Rats; Rats, Wistar; Transfection
- From: Chinese Journal of Applied Physiology 2004;20(2):156-160
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the protective effects of adenovirus-mediated hepatocyte growth factor (Ad-HGF) on injury of rat cortex neurons induced by in vitro serum-free culture.
METHODSFlow cytometry was used to assay the transfection rate of rat cortex neurons infected by adenovirus-mediated green fluorescent protein(Ad-GFP) at different multiplicity of infection (MOI) to find out the best MOI in experiment. ELISA was used to elucidate the expression patterns of cortex neuron. Neutral red stain and PI-Hoechst 33342 double stain were used to compare the viability of cortex neurons, which were cultured in serum-free medium for 6 h, 12 h, 24 h and 48 h respectively, among the Ad-HGF transfected group, the Ad-GFP transfected group and the control group.
RESULTSIt was found that when MOI was 50 PFU per cell, a transfection rate as high as 99.3% was maintained and Ad-HGF was able to express in cortex neurons effectively and persistently. In addition, the death rate and apoptotic rate of cortex neurons (infected 2 hours after seeding) cultured in serum-free medium for 12 h in Ad-HGF transfected group was significantly lower than that in both the Ad-GFP group and the control group (P < 0.05).
CONCLUSIONAd-HGF plays a protective role against in vitro serum-free culture induced injury on rat cortex neurons infected 2 hours after seeding. Though its effects on rat cortex neurons infected 5 days after seeding are not so remarkable, Ad-HGF also has the potential to protect cortex neurons from serum-free culture induced injury.