Expression of ryanodine receptor isoforms in airway smooth muscle cells of rats.
- Author:
Ying WANG
1
;
Sui-yang ZHANG
;
Xi-liang WANG
;
Gui-sheng QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Asthma; metabolism; Bronchi; Male; Myocytes, Smooth Muscle; metabolism; Protein Isoforms; genetics; metabolism; RNA, Messenger; genetics; Rats; Rats, Wistar; Ryanodine Receptor Calcium Release Channel; genetics; metabolism
- From: Chinese Journal of Applied Physiology 2004;20(2):181-184
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo detect the expression of ryanodine receptor (RyR) subtypes in normol rat airway smooth muscle cells(ASMCs) and changes during chronic asthma formation.
METHODSASMCs were cultured with collagen enzyme digestion method. The expression of subtypes of RyR were detected by RT-PCR. Purified PCR product linked with pGEM-T vector to make DNA sequence assay. Chronic asthma model was made with OVA, the changes of RyRs detected by RT-PCR.
RESULTSAll subtypes of RyR were expressed in airway smooth muscle cells of normol rat. The expression of RyR1 increased obviously compared with control group (P < 0.05) on chronic asthma.
CONCLUSIONCo-expression of three subtypes of RyR in ASMCs of normal rat, indicate that there are complicated intercellular Ca2+ regulation mechanism in ASM, moreover RyR1 might play a role during asthma development.
