Differentiation of human fetal liver CD34+ cells into neuronal cells induced by beta-ME and BHA in vitro.
- Author:
Ge-xiu LIU
1
;
Yuan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; Butylated Hydroxyanisole; pharmacology; Cell Differentiation; drug effects; Cells, Cultured; Hematopoietic Stem Cells; cytology; drug effects; Humans; Liver; cytology; embryology; Mercaptoethanol; pharmacology; Neurons; cytology
- From: Chinese Journal of Applied Physiology 2004;20(3):280-283
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish model of differentiation of fetal liver stem cells induced by beta-ME + BHA into neural cells in vitro;
METHODSCD34+ cells from naturally aborted human fetal liver were isolated with MACS Kit, and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS). After confluent more than 80%, the 5 passage cells were induced by 10(-3) mol/L beta-mercaptoethanol (beta-ME) and 2 x 10(-4) mol/L BHA for 24 hours, and washed with PBS, and then incubated in serum-free medium for 5 hours to 5 days. The characteristics of treated cells were assayed by immunocytochemistry staining analysis.
RESULTSCells treated by beta-ME+ BHA exhibited neuronal phenotype, and expressed neuronal specific proteins such as nestin, NeuN, TrnJ-1, and NF-M, which were not found in control cells. Statistic analysis showed that 81% cells were NeuN-positive, 75% cells TuJ-1-positive, 47% cells NF-M-positive, 90% cells NSE-positive.
CONCLUSIONbeta-ME + BHA can induce human fetal liver CD34+ cells to produce neuronal specific antigens and proteins in vitro and become neuronal cells. CD34+ cells from human fetal liver possess potentials of differentiation into neural cells.
