Testosterone has beneficial effects on human umbilical vein endothelial cells.
- Author:
Hong JIN
1
;
Lu FU
;
Yi-Fang MEI
;
Li-Jun ZHOU
;
Qian-Ping GAO
;
Jia LI
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Human Umbilical Vein Endothelial Cells; drug effects; metabolism; Humans; Nitric Oxide; metabolism; Plasminogen Activator Inhibitor 1; metabolism; Testosterone; pharmacology; Tissue Plasminogen Activator; metabolism
- From: Chinese Journal of Applied Physiology 2004;20(4):338-341
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the influences of testosterone with varied concentrations on the functions of HUVEC.
METHODSHuman umbilical vein endothelial cells within 2-3 passages were cultured with testosterone (3 x 10(-10) to 3 x 10(-8), 3 x 10(-6), 3 x 10(-5) mol/ L), and the control confluent cells were cultured in the same medium without steroid. MTT experiment was repeated for 7 days to investigate each groups' cell proliferation. The values of NO were tested as recommended. The tPA and PAI-1 antigen levels were assayed with ELISA Kits.
RESULTSTestosterone at physiologic or lower concentrations (3 x 10(-10) to 3 x 10(-8) mol/L ) had no adverse effect on A490 and NO level, meanwhile, stimulated the secretion of tPA (P < 0.01). However, tPA levels markedly reduced at larger dose (3 x 10(-6) to 3 x 10(-5) mol/L). On the other hand, PAI-1 antigen levels decreased significantly at the testosterone concentrations ranging from 3 x 10(-10) to 3 x 10(-5) mol/L (P < 0.05).
CONCLUSIONTestosterone at physiologically relevant concentrations affectively decreased PAI-1, while increased tPA levels, which suggested that testosterone might have beneficial effects on the Human umbilical vein endothelial cells and cardiovascular system to prevent atherosclerosis.
