The role of p38 on the differentiation of MSCs to myoblasts.
- Author:
Jin WANG
1
;
Cheng-Ji LUO
;
Xin-Ze RAN
;
Hui XU
;
Yong-Ping SU
;
Tian-Min CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Azacitidine; Bone Marrow Cells; cytology; Cell Differentiation; Cells, Cultured; Mesenchymal Stromal Cells; cytology; Mice; Mice, Inbred BALB C; Myoblasts; cytology; Myogenic Regulatory Factor 5; metabolism; Signal Transduction; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Chinese Journal of Applied Physiology 2004;20(4):345-348
- CountryChina
- Language:Chinese
-
Abstract:
AIMInducing mesenchymal stem cells (MSCs) differentiate to myoblasts with 5-azacytidine(5-Aza-CR), investigating the expression of Myf5 and the role of the signal transduction case of p38 in all the course of differentiation.
METHODSSeparating and purifying bone marrow-derived MSCs, inducing MSCs differentiation to myoblasts with 10 micromol/L 5-Aza-CR, assaying the gene expression time of Myf5 with RT-PCR method, the antigen expression of myosin with immunohistochemistry method and observing the changes of the activity of phosphorylation p38 before and after inhibited by SB203580 with Western-blot method.
RESULTSMSCs begin to express Myf5 delayed to the 9th day after inhibited by SB203580. Some of MSCs express myosin at the 7th day after induced; The phosphorylation p38 activity of MSCs enhanced after induced by 5-Aza-CR but obviously decreased after inhibited by SB203580.
CONCLUSIONMSCs can express myogenic regulator factors and orientation differentiate to myoblasts after induced by 5-Aza-CR, p38 really have a positive signal transduction affection in this course.
